UreB

基本解释

Methods BALB/c mice and rabbits were immunized with rUreB.The specific antibodies to UreB were analyzed by agarose diffusion, ELISA and Western blot.
方法重组尿素酶B亚单位注射免疫BALB/c小鼠、家兔,双向琼脂扩散、ELISA、免疫印迹分析特异抗体的产生及性质。
Recombinant pIRES ureB IL 2 and pIRES ureB were transfected into Cos 7 cells using LipofectAMINE TM 2000, the immunogenicity of UreB and IL 2 protein expressed was detected with SDS PAGE and Western blot.
通过脂质体法将重组载体 pIRES ureB和pIRES ureB IL 2转染Cos 7细胞 ,SDS PAGE及Westernblot法检测表达蛋白的免疫性。
Construct rice expression vector of CTB-ureB fusion gene pCWUN:(1) Fusion and clone of CTB gene and ureB gene: CTB gene and Hp ureB gene were amplified by high-fidelity PCR respectively from plasmid pGEM-CTB and Hp genome, and then they were fused by PCR.
构建CTB-ureB融合基因的水稻表达载体pCWUN：
Keywords Helicobacter pylori;DNA vaccine;UreB;HspA;
幽门螺杆菌;DNA疫苗;尿素酶;热休克蛋白A;
Keywords antigen epitope;Urease B （UreB）;antigenicity;identification;screening;fluorescence polarization （FP）;
抗原表位;尿素酶B;抗原性;鉴定;筛选;荧光偏振;