gene-splicing

常见例句

• Compared to traditional crossbreeding, gene splicing takes about half as much time to develop a new crop or animal variety.
  与传统的杂交育种相比，基因剪接约需多半的时间开发一种新的作物或动物品种。
• Scientists have used gene splicing to develop modified crop plants, new drugs, pest-resistant plants, and animals that grow rapidly.
  科学家们利用基因拼接开发改良作物，新的药物，抗虫植物和动物的迅速增长。
• Gene splicing 1.The joining of EXONS after the INTRON sequences have been removed, to produce functional messenger RNA that leaves the nucleus to undergo TRANSCRIPTION.
  基因拼接：1.;是将内含子序列剪切掉并使外显子相连接的过程，所形成的功能性信使RNA继续留在核中进一步转录。
• Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production.
  方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。
• Designed and synthesised 4 fragments of antibacterial peptide B gene, then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing).
  摘要人工设计并合成了抗菌肽B基因的4个寡聚核苷酸片段，通过重叠区扩增法，扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。
• Gene splicing by overlap extension, SOE
  作了改进,在拼接延伸后再进行一次
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